MAB3785

Anti-APC Antibody, NT, clone Ali 12.28

clone Ali 12.28, Chemicon^®, from mouse

• Synonym(s): Adenomatous Polyposis Coli Protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Ali 12.28, monoclonal
• species reactivity: human
• should not react with: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000038.3
• UniProt accession no.: P25054
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... APC(324) ; mouse ... Apc(11789)

Description

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Specificity

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Immunogen

Epitope: N-terminus

N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Linkage

Replaces: AB1268

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable