OP80

Anti-APC (Ab-7) Mouse mAb (CC-1)

• Synonym(s): Anti-Adenomatus Polyposis Coli
• UNSPSC Code: 12352203
• NACRES: NA.45

Properties

• biological source: mouse
• Quality Level: 100
• clone: CC-1, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (frozen sections): suitable
• dilution: (Free-floating SectionsParaffin Sections)
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recognizes APC in oligodendrocytes and astrocytes.

• Antibody Target Gene Symbol: APC

• Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
• Entrez Gene Name: adenomatous polyposis coli
• Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
• Mu Entrez ID: 11789
• RatEntrez ID: 24205

Immunogen

a recombinant protein consisting of amino acids 1-226 of APC

Application

Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)

Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration

Warning

Toxicity: Standard Handling

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative control: MOPC 21 cells

Positive control: Purkinje cells or cerebellum tissue

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable