IF005
Interferon-γ Protein, Recombinant mouse
The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.
Synonym(s):
IFN γ, IFN g, IFN gamma, INF γ, INF g, INF gamma
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
Recommended Products
biological source
mouse
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
form
liquid
specific activity
≥1.0 x 10E7 U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
input
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
shipped in
dry ice
General description
Interferon-gamma (IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+TCRalpha/beta+, CD8+TCRalpha/beta+, and TCRgamma/delta+ T cells. The biological activities of IFN-gamma include the specific binding to a single class of high affinity receptors. In addition, IFN-gamma can upgrade the number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. Recombinant Murine IFN-γ is a 134 amino-acid length protein with a predicted molecular weight of 15.6 kDa. C-terminal truncation due to proteolytic cleavage occurs during E. coli fermentation and results in a 15.1 kDa protein confirmed by mass spectroscopy. SDS-PAGE analysis of Recombinant Murine IFN-γ shows a band that migrates at approximately 11-12 kDa, under nonreducing and reducing conditions.
Product Source: Mouse, expressed in E.coli
Application
For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.
Biochem/physiol Actions
The ED50 as determined by a cytopathic effect inhibition assay with mouse NIH 3T3 cells challenged VSV is ≤ 0.1 ng/mL
Physical form
Sterile filtered, lyophilized from 10mM Sodium Phosphate, pH 7.0 + 25mM NaCl.
Storage and Stability
The lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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