AB5300

Anti-Amyloid Precursor Protein Antibody, universal

serum, Chemicon^®

• Synonym(s): APP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, monkey, mouse
• should not react with: bovine
• manufacturer/tradename: Chemicon^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_000484.2; NM_201413.1; NM_201414.1
• UniProt accession no.: P05067
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Description

Specificity

Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.

Immunogen

APP (aa 99-126) conjugated to KLH.

Epitope: universal

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.

Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope

Optimal working dilutions must be determined by end user.

Physical form

Serum. Liquid in PBS with 0.01% azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable