AB5302

Anti-Amyloid Precursor Protein Antibody, KPI Domain

serum, Chemicon^®

• Synonym(s): APP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: monkey, mouse, human, bovine
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_000484.2; NM_201413.1; NM_201414.1
• UniProt accession no.: P05067
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Description

Specificity

Amyloid Precursor Protein (APP), KPI Domain.

Immunogen

APP.

Application

Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.

Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.

Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.

Optimal working dilutions must be determined by end user.

Reduction /Alkylation protocol:

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable