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UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18 application for UHPLC

Materiais

acessório

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EXP® Pre-Column Filter

holder with EXP titanium hybrid ferrule, pack of 1 sets

EXP® Pre-Column Filter Cartridges

0.5μm, pkg of 5 ea

coluna analítica

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Coluna de HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

padrão

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Cannabidiol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Cannabinol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(−)-trans-Δ9-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(−)-Δ8-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Canabidivarina

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Canabigerol

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ácido canabigerólico

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Cannabichromene solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ácido canabidiólico

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Tetra-hidrocanabivarina (THCV)

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ácido delta9-tetraidrocanabinólico A

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Cannabidivarinic Acid (CBDVA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

Cannabichromenic Acid (CBCA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

Tetrahydrocannabivarinic acid (THCVA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 15 cm x 2.1 mm, 2.7 μm particles (53825-U)

mobile phase

[A] 0.1% formic acid in HPLC water; [B] 0.1% formic acid in acetonitrile; (30:70, A:B)

flow rate

0.6 mL/min

pressure

6390 psi (440 bar)

column temp.

20 °C

detector

UV, 230 nm

injection

2 μL

sample

25 μg/mL each compound in acetonitrile

Descrição

Nota de análise

Cannabinoids are a group of active compounds found in cannabis. They reportedly provide relief to an assortment of symptoms in the treatment of pain, nausea, anxiety, mood disorders, and inflammatory diseases. Cannabinoids imitate naturally occurring compounds and interact with two specific types of receptors: CB1 and CB2, located in the nervous system and immune system, respectively. Shown here is an isocratic separation of fourteen cannabinoids in less than twelve minutes on an Ascentis® Express C18 column. Cerilliant cannabinoid CRMs provide reliable identification and quantification. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany