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HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series application for HPLC

Materiais

coluna analítica

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Descrição
Preços

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

padrão

Nº do produto
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Preços

Sacarose

≥99.5% (GC)

Taurine

≥99%

D-(−)-Frutose

United States Pharmacopeia (USP) Reference Standard

D-(+)-Glicose

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

dilute1:9 in acetonitrile

Descrição

Nota de análise

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany