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HPLC Analysis of Steroids on Ascentis® Express F5

HPLC Analysis of Steroids on Ascentis® Express F5 application for HPLC

Materiais

coluna analítica

Nº do produto
Descrição
Preços

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

padrão

Nº do produto
Descrição
Preços

Prednisolone

≥99%

Hidrocortisona

≥98% (HPLC)

Prednisone

≥98%

CONDITIONS

column

Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 μm particles (53591-U)

mobile phase

[A] water; [B] methanol; (50:50, A:B)

flow rate

0.8 mL/min

pressure

2393 psi (165 bar)

column temp.

35 °C

detector

UV, 240 nm

injection

5 μL

sample

10 μg/mL of each in 90: 10, water: methanol

Descrição

Nota de análise

Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany