HPLC Analysis of Rabeprazole Enantiomers on Astec^® CYCLOBOND I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.4:0.1, A:B:C:D)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

1 mg/mL in methanol