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HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

Materiais

Padrão

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Preços

Sorbic acid

99.0-101.0% anhydrous basis

Nicotinamide (Niacinamide)

analytical standard

Riboflavina (B2)

analytical standard

SS Benzoic Acid

2000 μg/mL in dichloromethane, analytical standard

coluna analítica

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Preços

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

Descrição

Nota de análise

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany