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GC Analysis of FAMEs in Walnuts on SP®-2560

GC Analysis of FAMEs in Walnuts on SP®-2560 suitable for GC

Materiais

coluna analítica

Nº do produto
Descrição
Preços

Coluna de GC capilar SP®-2560

L × I.D. 100 m × 0.25 mm, df 0.20 μm

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Aldrich® single-neck round-bottom flask

capacity 50 mL, joint: ST/NS 24/40

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 37 mm, Overall H 270 mm

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 37 mm, Overall H 420 mm

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 54 mm, Overall H 300 mm

BF3 - Methanol

10 % (w/w)

CONDITIONS

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

180 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 1 mL/min

injection

1 μL, split 100:1

liner

4 mm ID FocusLiner w/taper

sample

ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09

Descrição

Nota de análise

This application illustrates the extraction and GC analysis of walnuts for fatty acids. Walnut is considered a significant plant source of alpha-linolenic acid (ALA). Both ALA and linoleic acid (LA) are the predominant essential fatty acids in humans (1). The samples were prepared using acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). BHT was added as an antioxidant prior to extraction. All samples were concentrated to 1 mL prior to GC analysis.
The FAME composition of vegetable oils is normally determined using AOCS Method Ce 1h-05, which designates the use of the a cyanopropylsilicone column (3). The SP-2560 column is specifically mentioned in the method. The use of a highly polar column enables simultaneous determination of trans isomers of unsaturated fatty acids along with saturated and cis-unsaturated.

Informações legais

SP is a registered trademark of Sigma-Aldrich Co. LLC