Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)

E Brindha, M Rajasekapandiyan

RESUMO

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIAIS

Número do produto

Marca

Descrição do produto

258148

Sigma-Aldrich

Ácido clorídrico, ACS reagent, 37%

320331

Sigma-Aldrich

Ácido clorídrico, ACS reagent, 37%

L3771

Sigma-Aldrich

Dodecilsulfato de sódio, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

345547

Sigma-Aldrich

Cloreto de hidrogênio, 4.0 M in dioxane

75746

Sigma-Aldrich

Dodecilsulfato de sódio, ≥99.0% (GC), dust-free pellets

S3014

Sigma-Aldrich

Cloreto de sódio, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

M1028

Sigma-Aldrich

Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M

H9892

Sigma-Aldrich

Ácido clorídrico, 1.0 N, BioReagent, suitable for cell culture

M8266

Sigma-Aldrich

Cloreto de magnésio, anhydrous, ≥98%

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

07102

Sigma-Aldrich

Ácido clorídrico, meets analytical specification of Ph. Eur., BP, NF, fuming, 36.5-38%

71736

Sigma-Aldrich

Dodecilsulfato de sódio, BioUltra, for molecular biology, 10% in H₂O

S5886

Sigma-Aldrich

Cloreto de sódio, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

339253

Sigma-Aldrich

Ácido clorídrico, 37 wt. % in H₂O, 99.999% trace metals basis

H1758

Sigma-Aldrich

Ácido clorídrico, 36.5-38.0%, BioReagent, for molecular biology

455180

Sigma-Aldrich

Cloreto de hidrogênio, 2.0 M in diethyl ether

30721

Sigma-Aldrich

Ácido clorídrico, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., fuming, ≥37%, APHA: ≤10

59222C

SAFC

Sodium chloride solution, 5 M

2104

Supelco

Ácido clorídrico, volumetric, 0.1 M HCl (0.1N), endotoxin free

05030

Sigma-Aldrich

Dodecilsulfato de sódio, BioUltra, for molecular biology, 20% in H₂O

71725

Sigma-Aldrich

Dodecilsulfato de sódio, BioUltra, for molecular biology, ≥99.0% (GC)

208337

Sigma-Aldrich

Cloreto de magnésio, powder, <200 μm

P9625

Sigma-Aldrich

Phenazine methosulfate

B4875

Sigma-Aldrich

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H₂O

204439

Sigma-Aldrich

Cloreto de sódio, 99.999% trace metals basis

294837

Sigma-Aldrich

Cloreto de hidrogênio, 1.0 M in diethyl ether

71376

Sigma-Aldrich

Cloreto de sódio, BioUltra, for molecular biology, ≥99.5% (AT)

68475

Sigma-Aldrich

Magnesium chloride solution, BioUltra, for molecular biology, 2 M in H₂O