- Modeling retinitis pigmentosa through patient-derived retinal organoids.
Modeling retinitis pigmentosa through patient-derived retinal organoids.
STAR protocols (2021-04-27)
Yan-Ping Li, Wen-Li Deng, Zi-Bing Jin
PMID33899019
RESUMO
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae. For complete details on the use and execution of this protocol, please refer to Deng et al. (2018).
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Sigma-Aldrich
Dimetilsulfóxido, Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture