ZRB2009

Anti-Sarcolipin Antibody, clone 1E10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): SLN
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 1E10, recombinant monoclonal
• descrição: 1E10 Clone
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt 3.76 kDa; observed mol wt ~12 kDa
• purificado por: using Protein A
• reatividade de espécies: rat, mouse
• reatividade da espécie (prevista por homologia): human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• sequência de epítopo: C-terminal
• nº de adesão de ID de proteína: NP_003054.1
• nº de adesão UniProt: O00631
• categoria alternativa mais ecológica: Aligned,
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SLN(6588)

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 1E10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Sarcolipin. It targets an epitope within the 6 amino acids from C-terminal, lumenal domain.

Imunogênio

KLH-conjugated linear peptide corresponding to 6 amino acids from the C-terminal region of human Sarcolipin.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Sarcolipin in C2C12 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sarcolipin in L6 cell lysate and 1:1,000 dilution detected Sarcolipin in Mouse heart tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound Sarcolipin peptide with a KD of 2.8 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Sarcolipin in Mouse heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descrição-alvo

Sarcolipin (UniProt: O00631; also known as SLN) is encoded by the SLN gene (Gene ID: 6588) in human. Sarcolipin is a single-pass membrane protein that contains a cytoplasmic domain (aa 1-7), a transmembrane domain (aa 8-26), and a lumenal domain (aa 27-31). SLN is a regulator of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in muscle and plays a role in regulating muscle thermogenesis and metabolism. It increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in muscle and contributes to non-shivering thermogenesis. This heat generating process is mechanistically distinct from and unrelated to Ca2+-linked thermogenic processes in skeletal muscle. It acts by reducing the apparent affinity of the ATPase for Ca2+. SLN is also reported to modulate calcium re-uptake during muscle relaxation and play an important role in calcium homeostasis in muscle. Higher levels of SLN have been reported in patients with Duchenne muscular dystrophy and reducing SLN levels improves dystrophic pathology. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Campbell, KL., and Dicke, AA (2018). Front. Physiol. 9; Article 714; Voit, A., et al. (2017). Nat. Commun. 8; Article 1068).

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1