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ZRB1709

Sigma-Aldrich

Anti-XIAP Antibody, clone 3H4 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinônimo(s):

E3 ubiquitin-protein ligase XIAP;EC:2.3.2.27;Baculoviral IAP repeat-containing protein 4;IAP-like protein;ILP;hILP;Inhibitor of apoptosis protein 3;IAP-3;hIAP-3;hIAP3;RING-type E3 ubiquitin transferase XIAP;X-linked inhibitor of apoptosis protein;X-linked

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

rabbit

Nível de qualidade

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

3H4, recombinant monoclonal

descrição

recombinant, expressed in HEK 293 cells

linha de produto

ZooMAb® learn more

forma

lyophilized

peso molecular

calculated mol wt 56.69 kDa
observed mol wt ~52 kDa

purificado por

using Protein A

reatividade de espécies

rat, monkey, human

embalagem

antibody small pack of 25 μL

características do produto alternativo mais ecológico

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validação aprimorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnica(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG

sequência de epítopo

N-terminal

nº de adesão de ID de proteína

nº de adesão UniProt

categoria alternativa mais ecológica

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... XIAP(331)

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 3H4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase XIAP. It targets an epitope within 24 amino acids from the N-terminal region.

Imunogênio

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human XIAP.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XIAP in COS-7 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected XIAP in C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected XIAP in A431 and HepG2 cells..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected XIAP in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound XIAP peptide with a KD of 5.0 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XIAP in COS-7 cell lysate.

Descrição-alvo

E3 ubiquitin-protein ligase XIAP (UniProt: P98170; also known as EC:2.3.2.27, Baculoviral IAP repeat-containing protein 4, IAP-like protein, ILP, hILP, Inhibitor of apoptosis protein 3, IAP-3, hIAP-3, hIAP3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein, X-linked IAP) is encoded by the XIAP (also known as API3, BIRC4, IAP3) gene (Gene ID: 331) in human. XIAP is one of the most potent anti-apoptotic IAP family member that contains three BIR domains (aa 26-93; 163-230; and 265-330). It is reported to directly neutralize caspase-9 via its BIR3 domain and the effector caspases-3 and -7 via its BIR2 domain. The BIR3 domain interacts with the Apaf-1/caspase-9 holoenzyme by sequestering the N-terminus of the small subunit of processed caspase-9. In addition to its role in regulating caspases and apoptosis, it also modulates inflammatory signaling and immunity, mitogenic kinase signaling, cell proliferation, and cell invasion and metastasis. It can directly bind to the active site pocket of caspase 3 and 7 and obstructs substrate entry. Aberrant expression of XIAP is reported in a variety of human cancers and it mediates cellular resistance to chemotherapeutic drugs. XIAP also acts as an E3 ubiquitin-protein ligase and regulates NF- B signaling. Other target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, MEKK2, DIABLO/SMAC, and Survivin. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Abbas, R., and Larisch, S. (2020). Cells. 9(3); 663; Obexer, P., and Ausserlechner, MJ. (2014). Front. Oncol. 4; 197).

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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