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ZMS1140

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone P4C10 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinônimo(s):

CD29, Fibronectin receptor subunit beta, GPIIA, Glycoprotein IIa, VLA-4 subunit beta

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

P4C10, recombinant monoclonal

linha de produto

ZooMAb® learn more

forma

lyophilized

peso molecular

calculated mol wt 88.42 kDa
observed mol wt ~150 kDa

purificado por

using protein G

reatividade de espécies

human

embalagem

antibody small pack of 25 μL

características do produto alternativo mais ecológico

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validação aprimorada

recombinant expression
Learn more about Antibody Enhanced Validation

técnica(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG2aκ

sequência de epítopo

N-terminal extracellular domain

nº de adesão de ID de proteína

nº de adesão UniProt

categoria alternativa mais ecológica

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... ITGB1(3688)

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone P4C10 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Integrin β1/CD29. It targets an epitope within 12 amino acids from the extracellular domain.

Imunogênio

Human fibrosarcoma HT-1080 cells.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate (non-reduced).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Integrin β1 in HeLa cell lysate (non-reduced).

Tested Applications

Flow Cytometry Analysis: 0.1 µg from a representative lot detected Integrin β1 in one million HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric integral membrane proteins composed of a variable α-subunit and a conserved β-subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various α and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the α-subunit. Integrin β1 is a single-pass type I membrane glycoprotein that associates with one of the a subunits to form receptor for fibronectin. It recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin β1 have been described that are produced by alternative splicing. Integrin β1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin β1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets, and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). (Ref.: Balcaz, A., et al. (1993). J. Cell Biol. 121(1); 171-178; Altruda, F., et al. (1990). Gene. 95(2); 261-266).

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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