U211000

DMT-2′O-TBDMS-rU Phosphoramidite

• Sinônimo(s): 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-uridine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-2′-O-TBDMS-rU amidite
• Fórmula empírica (Notação de Hill): C₄₅H₆₁N₄O₉PSi
• Número CAS: 118362-03-1
• Peso molecular: 861.05
• Número MDL: MFCD00080343
• Código UNSPSC: 12352200
• ID de substância PubChem: 329827595
• NACRES: NA.51

Propriedades

• fonte biológica: non-animal source (no BSE/TSE risk)
• Nível de qualidade: 300
• linha de produto: Proligo Reagents
• Ensaio: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Formulário: powder
• Impurezas: ≤0.3 wt. % water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3 wt. % residual Solvent content
• cor: white to off-white
• λ: conforms (UV/VIS Identity)
• adequação: conforms to structure for H-NMR; conforms to structure for LC-MS
• perfil de nucleosídeo: base: uridine; base protecting group: none; 2' protecting group: TBDMS; 5' protecting group: DMT; deprotection: fast/standard
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C45H61N4O9PSi/c1-31(2)49(32(3)4)59(55-29-15-27-46)57-40-38(56-42(48-28-26-39(50)47-43(48)51)41(40)58-60(10,11)44(5,6)7)30-54-45(33-16-13-12-14-17-33,34-18-22-36(52-8)23-19-34)35-20-24-37(53-9)25-21-35/h12-14,16-26,28,31-32,38,40-42H,15,29-30H2,1-11H3,(H,47,50,51)/t38-,40-,41-,42-,59?/m1/s1
• chave InChI: SKNLXHRBXYGJOC-ZMHKPELYSA-N

Descrição

Descrição geral

DMT-2′O-TBDMS-rU Phosphoramidite belongs to the group of 2′O-TBDMS RNA Phosphoramidites.

Aplicação

RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence-specific RNA oligonucleotides is a chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis.

Características e benefícios

• Industry-standard 2′O-TBDMS protective group
• Consistent lot-to-lot purity and performance
• Compatible with deprotection methods based on methylamine or AMA
• Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as activator; best results are obtained with Activator 42
• Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A
• Manufactured under a certified ISO 9001 quality system

Outras notas

The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as:

• Catalytic agents (ribozymes)
• Affinity ligands (aptamers)
• Agents to induce gene silencing (RNA interference)

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable