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Merck
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Key Documents

T1949

Sigma-Aldrich

Total Protein Reagent

Sinônimo(s):

Biuret Reagent

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About This Item

Código UNSPSC:
12352202
NACRES:
NC.07

Nível de qualidade

Aplicação

Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Total Protein Reagent has been used in the biuret assay.

Ações bioquímicas/fisiológicas

A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.

Pictogramas

CorrosionEnvironment

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificados de análise (COA)

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Determination of serum proteins by means of the biuret reagent.
Gornall, A. G., et al.,
The Journal of Biological Chemistry, 177, 759-759 (1949)
B T Doumas et al.
Clinical chemistry, 27(10), 1642-1650 (1981-10-01)
We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau
Claudia Einer et al.
Cellular and molecular gastroenterology and hepatology, 7(3), 571-596 (2018-12-27)
In Wilson disease, ATP7B mutations impair copper excretion into bile. Hepatic copper accumulation may induce mild to moderate chronic liver damage or even acute liver failure. Etiologic factors for this heterogeneous phenotype remain enigmatic. Liver steatosis is a frequent finding

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