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Key Documents

T1827

Sigma-Aldrich

Anti -TBP antibody, Mouse monoclonal

clone 58C9, purified from hybridoma cell culture

Sinônimo(s):

Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

58C9

peso molecular

antigen ~42 kDa

reatividade de espécies

Sf9 cell line, human, yeast, Drosophila melanogaster

técnica(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells

Isotipo

IgG2b

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Drosophila melanogaster ... Tbp(37476)
human ... TBP(6908)

Descrição geral

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).
TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

Imunogênio

Drosophila TFIID complex.

Aplicação

Monoclonal Anti-TBP antibody produced in mouse has been used in:
  • immunoblotting
  • co-immunoprecipitation
  • immunohistochemistry

Ações bioquímicas/fisiológicas

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.
The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

Descrição-alvo

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

PRP-1 significantly decreases the ALDHhigh cancer stem cell population and regulates the aberrant Wnt/beta-catenin pathway in human chondrosarcoma JJ012 cells
Hoyt AK, et al.
Oncology Reports, 42(1), 103-114 (2019)
Enhanced Human Decidual Cell-Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal
Schatz F, et al.
The American Journal of Pathology, 185(9), 2402-2411 (2015)
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.
Gouge J
Nature Communications, 8, 130-130 (2017)
TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family.
Rabenstein MD
Proceedings of the National Academy of Sciences of the USA, 96, 4791-4796 (1999)
SCA17, a novel autosomal dominant cerebellar ataxia caused by an expanded polyglutamine in TATA-binding protein.
Nakamura K, et.al.
Human Molecular Genetics, 10, 1441-1448 (2001)

Artigos

Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction, motor impairment and behavioral changes.

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