SRP7603

DNA Binding Protein-7 (DBP-7), recombinant

recombinant, expressed in E. coli, ≥99% (SDS-PAGE)

• Sinônimo(s): DNA Binding Protein 7
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• recombinante: expressed in E. coli
• Ensaio: ≥99% (SDS-PAGE)
• forma: lyophilized
• peso molecular: ~9.4 kDa
• embalagem: pkg of 20 μg
• Impurezas: endotoxin, tested
• nº de adesão NCBI: P39476
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... sso7d(1454006)

Descrição

Descrição geral

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

forma física

Sterile filtered and Lyophilized without additives.

Reconstituição

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable