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Key Documents

SRP6437

Sigma-Aldrich

CD55/DAF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sinônimo(s):

CR, CROM, TC

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (C-terminus)

Ensaio

≥95% (SDS-PAGE)

forma

lyophilized

peso molecular

calculated mol wt 35.8 kDa
observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)

embalagem

pkg of 10 μg
pkg of 50 μg

nº de adesão UniProt

P08174

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... CD55(1604)

Descrição geral

CD55 (complement decay-accelerating factor), also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells.

Ações bioquímicas/fisiológicas

CD55 (complement decay-accelerating factor) prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first short consensus repeats (SCR). It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C′ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration.
Rodrigues-Pinto R, et al.
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 23(9), 1803-1814 (2014)
Membrane complement regulatory proteins.
Kim D D and Song W C
Clinical Immunology (Orlando, Fla.), 118(2-3), 127-136 (2006)
Structure of complement fragment C3b?factor H and implications for host protection by complement regulators.
Wu J, et al.
Nature Immunology, 10(7), 728-728 (2009)
Hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (DAF/CD55).
Krautkramer E and Zeier M
Journal of Virology, 82(9), 4257-4264 (2008)
Molecular basis of reduced or absent expression of decay-accelerating factor in Cromer blood group phenotypes.
Lublin D M, et al.
Blood, 84(4), 1276-1282 (1994)
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