SRP6425

SERPIN A3 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinônimo(s): AACT, ACT, GIG24, GIG25
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: lyophilized
• peso molecular: calculated mol wt 46 kDa; observed mol wt 55-70 kDa (DTT-reduced. Protein migrates due to glycosylation. His 23 is the predicted N-terminal.)
• embalagem: pkg of 10 μg
• nº de adesão UniProt: P01011
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... SERPINA3(12)

Descrição

Descrição geral

Serpin A3 is also known as α-1-antichymotrypsin (ACT or AACT) gene with five exons and four introns spanning 12.8kb long, is mapped to human chromosome 14q32.13. The encoded protein is a 423 amino acid with the molecular weight of ~ 55-66kDa and it belongs to the family of serpin superfamily of protease inhibitors. Serpin A3 consists of three β sheets, eight α helices and an active site situated within a hypervariable reactive center loop (RCL).

Ações bioquímicas/fisiológicas

Overexpression of SERPIN A3 leads to endometrial carcinoma (EC), Therefore targeting SERPIN A3 can be a potent therapeutic method for EC. In addition, elevated expression of SERPIN A3 expression has been observed in various types of carcinomas including breast carcinoma, hepatocellular carcinoma, prostate carcinoma and adenocarcinoma of the lung. SERPINA3 is associated with the pathogenesis of chronic obstructive pulmonary disease (COPD), Parkinson′s disease (PD), Alzheimer′s disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy. SERPIN A3 inhibits the activity of pancreatic chymotrypsin, leukocyte, cathepsin G, mast cell chymases, human glandular, kallikrein 2, kallikrein 3 (prostate specific antigen), pancreatic cationic elastase and an uncharacterized lung serum protease. SERPIN A3 can bind to DNA, this property is unique among the members of SERPIN family.

forma física

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable