SRP3184

VEGF-C human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Sinônimo(s): VRP, Vascular Endothelial Growth Factor-C, flt4 ligand
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Formulário: lyophilized
• peso molecular: 30.0-33.0 kDa
• embalagem: pkg of 20 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white
• nº de adesão UniProt: P49767
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... VEGFC(7424)

Descrição

Descrição geral

Vascular endothelial growth factor-C (VEGF-C) is a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. It forms cell surface-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. Recombinant human VEGF-C is a 13.5kDa disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 30.0-33.0 kDa band by SDS-PAGE analysis under non-reducing condition.

Ações bioquímicas/fisiológicas

Vascular endothelial growth factor-C (VEGF-C) is a potent angiogenic factor. It promotes endothelial cell growth, lymphangiogesis and can also affect vascular permeability. VEGF-C can bind and activate both vascular endothelial growth factor receptors-2 and -3 (also known as flk1 and flt4 respectively). During embryogenesis, the protein may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis.

Sequência

AHYNTEILKS IDNEWRKTQC MPREVCIDVG KEFGVATNTF FKPPCVSVYR CGGCCNSEGL QCMNTSTSYL SKTLFEITVP LSQGPKPVTI SFANHTSCRC MSKLDVYRQV HSIIRR

forma física

Lyophilized with no additives.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable