SRP3020

sCD22 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): B-Lymphocyte cell adhesion molecule, BL-CAM, Leu-14, Siglec-2
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in CHO cells
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• forma: lyophilized
• potência: 10-17 μg/mL ED₅₀
• peso molecular: 75.0 kDa
• embalagem: pkg of 20 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white
• nº de adesão UniProt: P20273
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... CD22(933)

Descrição

Descrição geral

CD22 is a B-lineage restricted 135 kDa glycoprotein whose cell surface expression is limited to resting and activated B lymphocytes. The physiological role of CD22 is still unknown. Targeted disruption of CD22 in mice results in a reduced level of surface IgM on peripheral B cells suggesting a role for CD22 in limiting antigen receptor signaling. CD22 is a member of the Ig gene superfamily that uniquely binds a sialic acid-dependent ligand. Recombinant Human sCD22 is a soluble 75.0 kDa protein which corresponds to the extracellular domain of CD22.

Ações bioquímicas/fisiológicas

CD22 is a B-lineage restricted 135 kDa glycoprotein whose cell surface expression is limited to resting and activated B lymphocytes. Recombinant Human sCD22 is a soluble 75.0 kDa protein which corresponds to the extracellular domain of CD22.

Sequência

SKWVFEHPET LYAWEGACVW IPCTYRALDG DLESFILFHN PEYNKNTSKF DGTRLYESTK DGKVPSEQKR VQFLGDKNKN CTLSIHPVHL NDSGQLGLRM ESKTEKWMER IHLNVSERPF PPHIQLPPEI QESQEVTLTC LLNFSCYGYP IQLQWLLEGV PMRQAAVTST SLTIKSVFTR SELKFSPQWS HHGKIVTCQL QDADGKFLSN DTVQLNVKHT PKLEIKVTPS DAIVREGDSV TMTCEVSSSN PEYTTVSWLK DGTSLKKQNT FTLNLREVTK DQSGKYCCQV SNDVGPGRSE EVFLQVQYAP EPSTVQILHS PAVEGSQVEF LCMSLANPLP TNYTWYHNGK EMQGRTEEKV HIPKILPWHA GTYSCVAENI LGTGQRGPGA ELDVQYPPKK VTTVIQNPMP IREGDTVTLS CNYNSSNPSV TRYEWKPHGA WEEPSLGVLK IQNVGWDNTT IACARCNSWC SWASPVALNV QYAPRDVRVR KIKPLSEIHS GNSVSLQCDF SSSHPKEVQF FWEKNGRLLG KESQLNFDSI SPEDAGSYSC WVNNSIGQTA SKAWTLEVLY APRRLRVSMS PGDQVMEGKS ATLTCESDAN PPVSHYTWFD WNNQSLPHHS QKLRLEPVKV QHSGAYWCQG TNSVGKGRSP LSTLTVYYSP ETIGRR

forma física

Lyophilized from 1x PBS, pH 7.2.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable