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Key Documents

SRP0187

Sigma-Aldrich

Bcl-xL Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sinônimo(s):

Apoptosis regulator Bcl-X, BCL-XL/S, BCL2L1, Bcl-2-like protein 1

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥90% (SDS-PAGE)

forma

aqueous solution

peso molecular

28 kDa

embalagem

pkg of 100 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

concentração

>0.02 mg/mL

nº de adesão NCBI

Z23115

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... BCL2L1(598)

Descrição geral

BCL2L1 (B-cell lymphoma 2 like 1) gene codes for the anti-apoptotic protein called Bcl-xL (B-cell lymphoma-extra large) and its isoform Bcl-xS. Bcl-xS functions as an antagonist of Bcl-xL. This gene is localized to human chromosome 20q11.21.

Aplicação

Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Ações bioquímicas/fisiológicas

BCL2L1 (B-cell lymphoma 2 like 1) or Bcl-xS functions as a dominant negative regulator of the anti-apoptotic isoform Bcl-xL. Bcl-xL is involved in modulating anti-apoptotic capacity of chemotherapy-resistant tumors. This protein is highly expressed in michigan cancer foundation 7 (MCF7) human breast carcinoma cells, and studies show that overexpression of Bcl-xS in these MCF-7 cells results in heightened sensitivity to chemotherapy-induced apoptosis by agents such as VP-16 or taxol.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H360D

Classificações de perigo

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 2

Certificados de análise (COA)

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Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis.
Sumantran VN, et al.
Cancer Research, 55(12), 2507-2510 (1995)
Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL.
Nguyen HT, et al.
Molecular Human Reproduction, 20(2), 168-177 (2014)
Bcl-xL mediates therapeutic resistance of a mesenchymal breast cancer cell subpopulation.
Keitel U, et al.
Oncotarget, 5(23), 11778-11791 (2014)
BCL-XL mediates the strong selective advantage of a 20q11.21 amplification commonly found in human embryonic stem cell cultures.
Avery S, et al.
Stem Cell Reports, 1(5), 379-386 (2013)
Pawel Licznerski et al.
Cell, 182(5), 1170-1185 (2020-08-17)
Loss of the gene (Fmr1) encoding Fragile X mental retardation protein (FMRP) causes increased mRNA translation and aberrant synaptic development. We find neurons of the Fmr1-/y mouse have a mitochondrial inner membrane leak contributing to a "leak metabolism." In human
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