SAMMS2HYGV
MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352200
NACRES:
NA.51
Produtos recomendados
embalagem
pkg of 8x25 μL (aliquots supplied at a minimum p24 titer of 1x106 VP/mL)
concentração
≥1x106 VP/ml (via p24 assay)
aplicação(ões)
CRISPR
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Descrição geral
Ready to use MS2-P65-HSF1 lentiviral particles enable immediate transduction of a wide range of cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a three part CRISPR system with individual dCas9-VP64. MS2-p65-HSF1 and gRNA expression vectors.
To order gRNA in any format click here
To order gRNA in any format click here
Aplicação
Functional Genomics/Target Validation
- Unbiased forward genetic screening
- Strong transcriptional activation in multiple cell lines
- Creation of cell lines stably expressing MS2-p65-HSF1
Características e benefícios
- Highly specific and highly active
- Sequence verified high purity, high titer lentiviral particles
- Activates genes through transcriptional activation rather than cDNA based overexpression
Learn more about SAM CRISPR Activators at SigmaAldrich.com/CRISPRa
Princípio
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be rendered inactive (dCas9) with mutations to the two protein domains, RuvC and HnH (D10A and H840A respectively), which are responsible for nuclease activity. The nuclease deficient protein can then be fused with the transcriptional activator VP64 and used in conjunction with a guide RNA modified with MS2 RNA aptamers that function to recruit the additional transcriptional coactivators p65 and HSF1. The assembled SAM complex is then used as a cargo delivery system to target gene promoters, enabling site-specific transcriptional activation of the gene of interest.
Definição da unidade
VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann S, et al
Nature, 517, 583-588 (2015)
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann S, et al.
Nature, 517, 583-588 (2015)
Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening.
Joung, S. et al.
Nature Protocols, 12, 828-863 (2017)
Julia Joung et al.
Nature protocols, 12(4), 828-863 (2017-03-24)
Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats) immune system
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica