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SAB4600052

Sigma-Aldrich

Anti-Chicken IgY (H+L), CF 488A antibody produced in rabbit

~2 mg/mL, affinity isolated antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

conjugado

CF 488A conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

reatividade de espécies

chicken

concentração

~2 mg/mL

técnica(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL

fluorescência

λex 490 nm; λem 515 nm

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Immunoglobulin Y is one of the antibodies produced in birds. It is generally related to mammalian IgG. However, IgY has different characteristics compared to mammalian IgG, for example, IgY does not bind with mammalian complements nor Rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. These characteristics of IgY is beneficial when it is used as a biochemical/clinical reagent. Immunoglobulin Y, present in chicken blood serum, has the capability to pass across the follicular epithelium of the ovary and aggregate in the yolk during oogenesis. Structurally IgY and mammalian IgG differ in the heavy chain with the molecular weight of 68,000 and 50,000 respectively. Mammalian IgG has slightly higher stability compared to IgY.
CF488A is a green fluorescent dye with excitation argon laser line at 488 nm. CF488A is minimally charged, thus it reduces non-specific binding of antibody conjugates. CF488A is a highly water-soluble and pH-insensitive dye with molecular weight of ~710. This dye has absorption/emission maxima at 490/515 nm and it is highly photostable.

Especificidade

Binds all chicken IgYs

Imunogênio

chicken IgY (H+L)

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

forma física

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Nota de preparo

Protect from light.

Informações legais

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Molecular stability of chicken and rabbit immunoglobulin G.
Shimizu M
Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274 (1992)
Immunoglobulins of the non-galliform birds: antibody expression and repertoire in the duck.
Lundqvist ML
Developmental and Comparative Immunology, 30(1-2), 93-100 (2006)

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