SAB4200753
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal
clone AK-11, purified from hybridoma cell culture
Sinônimo(s):
Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.44
clone:
AK-11, monoclonal
application:
IF
IP
IP
reatividade de espécies:
mouse, chicken, rat, human
técnica(s):
immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
citations:
4
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified from hybridoma cell culture
tipo de produto de anticorpo
primary antibodies
clone
AK-11, monoclonal
Formulário
buffered aqueous solution
peso molecular
~56 kDa
reatividade de espécies
mouse, chicken, rat, human
concentração
~1.0 mg/mL
técnica(s)
immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
Isotipo
IgG2a
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
phosphorylation (pThr450)
Informações sobre genes
human ... AKT1(207)
Descrição geral
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.
Especificidade
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).
Imunogênio
Synthetic peptide corresponding to human AKT1 pThr450
Aplicação
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunoprecipitation
Ações bioquímicas/fisiológicas
Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Armazenamento e estabilidade
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Exoneração de responsabilidade
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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H Dudek et al.
Science (New York, N.Y.), 275(5300), 661-665 (1997-01-31)
A signaling pathway was delineated by which insulin-like growth factor 1 (IGF-1) promotes the survival of cerebellar neurons. IGF-1 activation of phosphoinositide 3-kinase (PI3-K) triggered the activation of two protein kinases, the serine-threonine kinase Akt and the p70 ribosomal protein
Zhi-Ping Yang et al.
World journal of gastroenterology, 21(36), 10385-10399 (2015-10-01)
To investigate the anti-tumor effects of equol in gastric cancer cells and the underlying molecular mechanisms. MGC-803 cells were employed for in vitro experiments in this study. Cells were treated with control (vehicle, 0.1% DMSO) or equol under specified dose
Won Jun Oh et al.
The EMBO journal, 29(23), 3939-3951 (2010-11-04)
The mechanisms that couple translation and protein processing are poorly understood in higher eukaryotes. Although mammalian target of rapamycin (mTOR) complex 1 (mTORC1) controls translation initiation, the function of mTORC2 in protein synthesis remains to be defined. In this study
A Khwaja et al.
The EMBO journal, 16(10), 2783-2793 (1997-05-15)
Upon detachment from the extracellular matrix, epithelial cells enter into programmed cell death, a phenomenon known as anoikis, ensuring that they are unable to survive in an inappropriate location. Activated ras oncogenes protect cells from this form of apoptosis. The
L Xiao et al.
Cell death and differentiation, 17(9), 1448-1462 (2010-02-27)
AKT pathway has a critical role in mediating signaling transductions for cell proliferation, differentiation and survival. Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases
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