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Key Documents

SAB4200749

Sigma-Aldrich

Anti-Collagen Type III antibody, Mouse monoclonal

clone FH-7A, purified from hybridoma cell culture

Sinônimo(s):

Anti-COL3A1, Anti-Collagen alpha-1(III) chain

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

FH-7A, monoclonal

forma

buffered aqueous solution

peso molecular

~70 kDa

reatividade de espécies

rat, human

concentração

~1.0 mg/mL

técnica(s)

ELISA: suitable
immunoblotting: suitable
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections.

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... COL3A1(1281)

Descrição geral

Anti-collagen type III antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.
Monoclonal anti-collagen type III specifically recognizes native and denatured collagen type III from human 1 and rat 2 origin. It does not recognize collagen types I, II, IV, V, VI and X.
Collagen type III, also known as collagen α-1(III) (COL3A1), is encoded by the gene mapped to human chromosome 2q24.3-q31. It is the highly expressed collagen in blood vessels and hollow organs.

Imunogênio

Human Collagen Type III

Ações bioquímicas/fisiológicas

Collagen a-1(III) (COL3A1) serves as a potent adjunct marker for both differentiating fibroadenoma (FA) from phyllodes tumor (PT) and measuring malignant potential in PTs. Overexpression of the gene in glomerular leads to the development of collagen type III glomerulopathy. Mutations in the gene is associated with the development of familial aortic aneurysms and Ehlers-Danlos syndrome.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Outras notas

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

The extracellular matrix at a glance.
Christian Frantz et al.
Journal of cell science, 123(Pt 24), 4195-4200 (2010-12-03)
Linkage mapping of the gene for type III collagen (COL3A1) to human chromosome 2q using a VNTR polymorphism.
Tiller GE, et al.
Genomics, 20, 275-277 (1994)
Glomerular Collagen V Codeposition and Hepatic Perisinusoidal Collagen III Accumulation in Canine Collagen Type III Glomerulopathy.
R?rtveit R, et al.
Veterinary Pathology, 52, 1134-1141 (2015)
Marion K Gordon et al.
Cell and tissue research, 339(1), 247-257 (2009-08-21)
The collagens represent a family of trimeric extracellular matrix molecules used by cells for structural integrity and other functions. The three alpha chains that form the triple helical part of the molecule are composed of repeating peptide triplets of glycine-X-Y.
Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.
Chiarelli N, et al.
PLoS ONE, 13 (2018)

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