SAB4200473

Anti-EpCAM ICD antibody, Mouse monoclonal

clone 4A7, purified from hybridoma cell culture

• Sinônimo(s): Monoclonal Anti-DIAR5, Monoclonal Anti-EGP-2, Monoclonal Anti-EGP314, Monoclonal Anti-EGP40, Monoclonal Anti-ESA, Monoclonal Anti-EpCAM ICD antibody produced in mouse, Monoclonal Anti-HNPCC8, Monoclonal Anti-KS1/4, Monoclonal Anti-KSA, Monoclonal Anti-M4S1, Monoclonal Anti-MIC18, Monoclonal Anti-MK-1, Monoclonal Anti-TACSTD1, Monoclonal Anti-TROP1, Monoclonal Anti-epithelial cell adhesion molecule
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: 4A7, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~40 kDa
• reatividade de espécies: human, mouse
• concentração: ~1.0 mg/mL
• técnica(s): indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.; western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.
• Isotipo: IgG1
• nº de adesão UniProt: P16422
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... EPCAM(4072) ; mouse ... Epcam(17075)

Descrição

Descrição geral

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

Imunogênio

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Aplicação

Monoclonal Anti-EpCAM ICD antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence

Ações bioquímicas/fisiológicas

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable