SAB4200400

Anti-WIPI-2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Sinônimo(s): Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WD repeat domain, phosphoinositide interacting 2, Anti-WIPI2
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~49 kDa
• reatividade de espécies: mouse, rat, human
• concentração: ~1.0 mg/mL
• técnica(s): immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.
• nº de adesão UniProt: Q9Y4P8
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... WIPI2(26100) ; mouse ... Wipi2(74781) ; rat ... Wipi2(288498)

Descrição

Descrição geral

WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.

Imunogênio

synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Aplicação

Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including

• immunoblotting
• immunoprecipitation
• immunostaining.

Ações bioquímicas/fisiológicas

WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable