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Key Documents

SAB4200310

Sigma-Aldrich

Anti-Granulin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinônimo(s):

Anti-Acrogranin, Anti-Epithelin, Anti-GEP, Anti-GP88, Anti-GRN, Anti-PCDGF, Anti-PEPI, Anti-PGRN, Anti-Progranulin

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About This Item

Código UNSPSC:
51111800
NACRES:
NA.41

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~80 kDa

reatividade de espécies

human

concentração

~1.0 mg/mL

técnica(s)

indirect immunofluorescence: 10-20 μg/mL using A431 cells
western blot: 1-2 μg/mL using MCF7 cell extracts

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GRN(2896)

Categorias relacionadas

Descrição geral

Granulin is composed of 7.5 repeat units of a unique, structurally defined cysteine-rich granulin-eptihelin motif.
Granulins (also known as granulin/epithelin precursor [GEP]) are a secreted growth factors expressed mainly by granulocytes. They are secreted as high molecular weight precursor proteins, pro-granulins (PGRN), with seven and a half repeats of 12-cysteine granulin motif. Five human granulins have been isolated and they have a common precursor. PGRN is highly expressed in rapidly proliferating epithelial cells such as keratinocytes. It is also synthesised in neurons of cerebral cortex, hippocampus and cerebellum, central and peripheral nervous system.

Imunogênio

synthetic peptide corresponding to a sequence at the C-terminus of human granulin precursor, conjugated to KLH.

Aplicação

Anti-Granulin (C-terminal) antibody produced in rabbit has been used in immunoblotting, immunofluorescence and western blotting.

Ações bioquímicas/fisiológicas

Granulins are potent mitogenic factors, and mediate proliferation, motility, wound healing and anchorage-dependent growth of fibroblasts. Some of the pathways that are associated with PGRN are MAPK and PI3K/Akt.
Progranulin gene is mitotically quiescent epithelia and is expressed in low levels. It stimulates the proliferation in epithelial cells and promotes their anchorage-independent growth. Overexpression of progranulin confers epithelial invasiveness and tumorigenicity. There is growing evidence that granulin is involved in embryonic and neonatal development, placenta, the epidermis, vasculature angiogenesis, and the developing nervous system. Granulin stimulates an increase in cyclins D1 and cyclins B. Granulin overexpression has been associated with many cancers, including glioblastoma, breast, renal and invasive ovarian cancers. Granulin is highly expressed in motor neurons, it regulates neurite outgrowth and promotes neuronal cell survival. Progranulin gene mutations and PGRN gene haploinsufficiency have been recently proposed to cause a form of frontotemporal dementia (FTLD). It is also associated with the formation of pathogenic Tar-DNA Binding Protein 43 (TDP-43) and ubiquitin positive inclusions.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Progranulin (acrogranin/PC cell-derived growth factor/granulin-epithelin precursor) is expressed in the placenta, epidermis, microvasculature, and brain during murine development
Daniel R, et al.
Developmental Dynamics, 227(4), 593-599 (2003)
Progranulin is expressed within motor neurons and promotes neuronal cell survival
Ryan CL, et al.
BMC Neuroscience, 10(1), 130-130 (2009)
T Zanocco-Marani et al.
Cancer research, 59(20), 5331-5340 (1999-10-28)
Growth-regulated cells, such as 3T3 mouse embryo fibroblasts (MEFs), require more than one growth factor for growth, usually the insulin-like growth factor I (IGF-I) in combination with either platelet-derived growth factor or epidermal growth factor. Singly, these growth factors cannot
Cellular localization of gene expression for progranulin
Daniel R, et al.
The Journal of Histochemistry and Cytochemistry, 48(7), 999-1009 (2000)
Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients
Valdez C, et al.
Human Molecular Genetics, 26(24), 4861-4872 (2017)

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