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Key Documents

SAB3700420

Sigma-Aldrich

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Sinônimo(s):

Anti-Pig IgG F(ab′)2-Peroxidase antibody produced in rabbit, Anti-Porcine IgG F(ab′)2-Peroxidase antibody produced in rabbit, HRP

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

rabbit

conjugado

peroxidase conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

forma

lyophilized powder

reatividade de espécies

swine

técnica(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Immunoglobulin G (IgG) is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.

Especificidade

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Swine IgG F(ab′)2 and Swine Serum. No reaction was seen against Swine IgG F(c) .

Imunogênio

Swine IgG F(ab′)2 fragment

Aplicação

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).(3)

propriedades físicas

Antibody format: IgG

forma física

Supplied in 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstituição

Reconstitute with 1.0 mL deionized water (or equivalent).

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Antibody structure, instability, and formulation
Immunobiology: The Immune System in Health and Disease, 96(1), 1-26 (2001)
Lycopene affects the immune responses of finishing pigs
Marcelise RF, et al.
Italian Journal of Animal Science, 17(3), 666-674 (2018)

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