RAB0436

Human Osteopontin ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Sinônimo(s): OPN
• Código UNSPSC: 41116158
• NACRES: NA.32

Propriedades

• reatividade de espécies: human
• embalagem: kit of 96 wells (12 strips x 8 wells)
• técnica(s): ELISA: suitable; capture ELISA: suitable
• entrada: sample type serum; sample type plasma; sample type urine; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 50 pg/mL; standard curve range: 74.07-18000 pg/mL
• método de detecção: colorimetric
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... SPP1(6696)

Descrição

Descrição geral

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Imunogênio

Recombinant Human OPN

Aplicação

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human Osteopontin ELISA Kit has been used in the quantification of serum osteopontin (OPN) in human blood samples.

Ações bioquímicas/fisiológicas

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to α_vβ₃ integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

Outras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Componentes do kit também disponíveis separadamente

• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Warning
• Frases de perigo: H290
• Declarações de precaução: P234 - P390
• Classificações de perigo: Met. Corr. 1
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials