R5028

Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse

clone 4F4, purified immunoglobulin, buffered aqueous solution

• Sinônimo(s): Anti-Heterogeneous Nuclear Ribonucleoprotein-C1/C2
• Número MDL: MFCD06798703
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4F4, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen 41 kDa; antigen 43 kDa
• reatividade de espécies: monkey, human, chicken, hamster
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using HeLa cell nuclear extract
• Isotipo: IgG1
• nº de adesão UniProt: P07910
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HNRNPC(3183)

Descrição

Descrição geral

Monoclonal Anti-hnRNP-C1/C2 (mouse IgG1isotype) is derived from the 4F4 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with RNPs eluted from oligo (dT) cellulose column. Heterogeneous nuclear ribonucleoproteins C1/C2 (HnRNP C1/C2) are synthesized from C2 protein by alternative splicing and have an additional13-amino-acids. It is localized in nucleus and has leucine zipper-like RNA-binding motif. The hnRNP-C proteins have a single ribonucleoprotein (RNP) motif RNA-binding domain (RBD) of 80 to 100 amino acid long.

Imunogênio

RNPs eluted from oligo (dT) cellulose column.

Aplicação

Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse has been used in:

• antibody microarray
• western blotting
• enzyme linked immunosorbent assay (ELISA)`
• immunoprecipitation
• immunocytochemistry

Ações bioquímicas/fisiológicas

Heterogeneous nuclear ribonucleoproteins C1/C2(HnRNP C1/C2) is involved in protein translation, mRNA biogenesis and transport. The hnRNP-C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding. Mutation of the hnRNPC gene is implicated in embryonic lethal phenotype.

forma física

Solution in phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable