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Documentos Principais

P9827

Sigma-Aldrich

Polycytidylic acid–Agarose

lyophilized powder

Sinônimo(s):

Poly(C)–Agarose potassium salt

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About This Item

Número MDL:
Código UNSPSC:
41106500
NACRES:
NA.56

forma

lyophilized powder

Nível de qualidade

Extensão da rotulagem

0.25-1.0 mg per mL

matriz

cross-linked 4% beaded agarose

ativação da matriz

cyanogen bromide

ligação da matriz

amino

espaçador de matriz

1 atom

inchaço

1 g swells to ~5 mL

temperatura de armazenamento

−20°C

Aplicação

Polycytidylic acid-agarose is used in protein chromatography, affinity chromatography and specialy resins. Polycytidylic acid has been used to study the preferential induction of apoptosis in the rainbow trout macrophage cell line. Polycytidylic acid has also been used to develop two analytical methods for human secretory-type ribonuclease.

forma física

Lyophilized powder stabilized with lactose

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Preparation of sepharose-bound poly (rI:rC).
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Fish & shellfish immunology, 18(4), 279-295 (2004-11-25)
The rainbow trout macrophage cell line RTS11 was found to be considerably more sensitive than rainbow trout fibroblast (RTG-2) and Chinook salmon epithelial (CHSE-214) cell lines to killing by macromolecular synthesis inhibitors, actinomycin D (AMD) and cycloheximide (CHX), a synthetic
D Nadano et al.
Analytical biochemistry, 212(1), 111-116 (1993-07-01)
Two analytical methods for human secretory-type ribonuclease, which are based on polycytidylic acid/ethidium bromide fluorescence, have been developed. The first is a method for measurement of secretory-type ribonuclease activity utilizing the radial diffusion of ribonuclease in a thin agarose gel
W R Paulding et al.
The Journal of biological chemistry, 274(4), 2532-2538 (1999-01-16)
The stability of mRNA for tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis, is regulated by oxygen tension in the pheochromocytoma-derived PC12 cell line. We previously identified a pyrimidine-rich 27-base-long protein-binding sequence in the 3'-untranslated region of TH mRNA

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