Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

P6248

Sigma-Aldrich

Anti-Parkin antibody, Mouse monoclonal

clone PRK8, purified from hybridoma cell culture

Sinônimo(s):

Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD02263896
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

PRK8, monoclonal

forma

buffered aqueous solution

reatividade de espécies

mouse, rat, hamster, human

concentração

~2 mg/mL

técnica(s)

microarray: suitable
western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract

Isotipo

IgG2b

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)

Descrição geral

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.
Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

Imunogênio

recombinant human Parkin.

Aplicação

Monoclonal anti-parkin antibody has been used in:
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunoblotting
  • Immunoprecipitation[63}
  • Immunocytochemistry
  • to label parkin in pull-down assay

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Ferramenta de seleção de produtos.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Genomic organization and expression of parkin in Drosophila melanogaster
Bae JY, et al.
Experimental & Molecular Medicine, 35(5), 393-393 (2003)
Parkin regulates kainate receptors by interacting with the GluK2 subunit
Maraschi AM, et al,
Nature Communications, 5(46) (2014)
Victoriane Peugnet et al.
Antioxidants (Basel, Switzerland), 11(4) (2022-04-24)
Heart failure, mostly associated with cardiac hypertrophy, is a major cause of illness and death. Oxidative stress causes accumulation of reactive oxygen species (ROS), leading to mitochondrial dysfunction, suggesting that mitochondria-targeted therapies could be effective in this context. The purpose
Koji Yamano et al.
The Journal of biological chemistry, 290(42), 25199-25211 (2015-08-12)
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1
Jérôme Piquereau et al.
Autophagy, 9(11), 1837-1851 (2013-10-15)
Mitochondrial quality control plays a vital role in the maintenance of optimal mitochondrial function. However, its roles and regulation remain ill-defined in cardiac pathophysiology. Here, we tested the hypothesis that PARK2/Parkin, an E3-ligase recently described as being involved in the
Exibir todos os documentos relacionados

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica