P5431
Pectolyase from Aspergillus japonicus
aseptically filled, lyophilized powder, ≥2 units/mg solid
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About This Item
esterilidade
aseptically filled
forma
lyophilized powder
atividade específica
≥2 units/mg solid
temperatura de armazenamento
2-8°C
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Aplicação
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Outras notas
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Definição da unidade
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
forma física
Lyophilized powder containing lactose
Nota de preparo
Prepared from P 3026
produto comparável
Nº do produto
Descrição
Preços
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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International microbiology : the official journal of the Spanish Society for Microbiology, 15(1), 1-7 (2012-07-28)
Macrophomina phaseolina, phylum Ascomycota, is a phytopathogenic fungus distributed worldwide in hot dry areas. There are few studies on its secreted lipases and none on its colony radial growth rate, an indicator of fungal ability to use nutrients for growth
Protein expression and purification, 87(1), 11-16 (2012-10-17)
Polygalacturonases are the pectinolytic enzymes that catalyze the hydrolytic cleavage of the polygalacturonic acid chain. In the present study, polygalacturonase from Aspergillus niger (MTCC 3323) was purified. The enzyme precipitated with 60% ethanol resulted in 1.68-fold purification. The enzyme was
The Analyst, 137(20), 4855-4859 (2012-09-05)
Polygalacturonases (EC 3.2.1.15) hydrolyze the α-1,4-glycosidic linkages in polygalacturonic acid chains. The interest on specific inhibitors of pectinase and the versatility of magnetic support for enzyme immobilization endorsed the preparation of an immobilized enzyme reactor (IMER). This work presents the
Genetics and molecular research : GMR, 11(3), 3091-3104 (2012-09-26)
Molecular chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly expanded multiple cloning site with an adjacent
BMC plant biology, 12, 150-150 (2012-08-28)
Cell separation that occurs during fleshy fruit abscission and dry fruit dehiscence facilitates seed dispersal, the final stage of plant reproductive development. While our understanding of the evolutionary context of cell separation is limited mainly to the eudicot model systems
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