P0073
Anti-PERK (N-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinônimo(s):
Anti-E2AK3, Anti-EIF2AK3, Anti-HsPEK, Anti-PEK, Anti-PRKR-like endoplasmic reticulum kinase, Anti-Pancreatic eIF2α kinase, Anti-WRS
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
conjugado:
unconjugated
application:
WB
clone:
polyclonal
reatividade de espécies:
mouse, rat
citations:
7
técnica(s):
western blot: 1:500-1:1,000 using whole cell lysates of HEK-293T expressing PERK
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
IgG fraction of antiserum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
Formulário
buffered aqueous solution
reatividade de espécies
mouse, rat
técnica(s)
western blot: 1:500-1:1,000 using whole cell lysates of HEK-293T expressing PERK
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
mouse ... Eif2ak3(13666)
rat ... Eif2ak3(29702)
Descrição geral
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is one of the three mammalian UPR (unfolded protein response) transducers; the other two being activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1). It is a highly conserved eIF2a (eukaryotic initiation factor 2 α) kinase.
Especificidade
Anti-PERK (N-terminal) specifically recognizes mouse and rat PERK.
Imunogênio
synthetic peptide corresponding to amino acids 124-139 of mouse PERK, conjugated to KLH. This sequence is identical in rat and mouse.
Aplicação
Anti-PERK (N-terminal) antibody produced in rabbit may be used in immunoblotting.
Ações bioquímicas/fisiológicas
Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). This results in reduction of protein load in the ER lumen. In S63del-Charcot-Marie-Tooth 1B neuropathy mice model, the suppression of PERK results in improved myelination. In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. Administration of GSK2606414, a PERK inhibitor, results in suppression of tau-mediated neurodegeneration. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) in humans, which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation, and osteopenia.
Descrição-alvo
PERK (also known as eIF2AK3, PEK and WRS) is a transmembrane kinase that is highly expressed in thepancreas. It resides in the ER and couples stress signals initiated by protein malfolding in the ER lumen to eIF2α phosphorylation and reduces protei
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Armazenamento e estabilidade
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Perk gene dosage regulates glucose homeostasis by modulating pancreatic ?-cell functions.
Wang R et al
PLoS ONE, 9(6), e99684-e99684 (2014)
Perk Ablation Ameliorates Myelination in S63del-Charcot-Marie-Tooth 1B Neuropathy.
Musner N et al
ASN Neuro, 8(2) (2016)
PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia.
Radford H et al
Acta Neuropathologica, 130(5), 633-642 (2015)
The eIF2a kinase PERK limits the expression of hippocampal metabotropic glutamate receptor-dependent long-term depression.
Trinh MA et al
Learning & Memory, 21(5), 298-304 (2014)
Mohamad A Zaini et al.
Scientific reports, 7, 42603-42603 (2017-02-16)
An important part of the beneficial effects of calorie restriction (CR) on healthspan and lifespan is mediated through regulation of protein synthesis that is under control of the mechanistic target of rapamycin complex 1 (mTORC1). As one of its activities
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