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N2415

Sigma-Aldrich

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

Sinônimo(s):

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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About This Item

Número CAS:
9030-21-1
Número da licença da enzima:
2.4.2.1 (BRENDA, IUBMB)
Número CE:
232-857-3
Número MDL:
MFCD00131739
Código UNSPSC:
12352204
NACRES:
NA.54

recombinante

expressed in E. coli

Nível de qualidade

200

Ensaio

≥80% protein basis (biuret)

forma

lyophilized powder

atividade específica

≥10 units/mg protein

observação sobre a sequência

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

nº de adesão UniProt

P0ABP8

temperatura de armazenamento

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

chave InChI

MRWXACSTFXYYMV-FDDDBJFASA-N

Informações sobre genes

Escherichia coli ... deoD(945654)

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Aplicação

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Ações bioquímicas/fisiológicas

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Definição da unidade

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Nota de preparo

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Primary T-cell immunodeficiencies
Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)
Disorders of purines and pyrimidines
Handbook of Clinical Neurology, 827-838 (2014)
Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Johnston S B and Raines R T
Biochemistry, 54(7), 1576-1582 (2015)
Patricia Calero et al.
Nature communications, 11(1), 5045-5045 (2020-10-09)
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and
Sean B Johnston et al.
PloS one, 10(1), e0116898-e0116898 (2015-01-22)
Phosphatase and tensin homologue deleted from chromosome ten (PTEN) is a lipid phosphatase tumor suppressor that is lost or inactivated in most human tumors. The enzyme catalyzes the hydrolysis of phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) to form phosphatidylinositol-(4,5)-bisphosphate (PIP2) and inorganic phosphate. Here
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