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MTOXRTSUP

Sigma-Aldrich

RPTEC Tox Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

Nível de qualidade

300

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 6 mL of RPTEC Tox media supplement which is added to basal media and other components listed below to produce 500 mL of media needed for optimal culture conditions of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC) when used for Kidney Toxicology studies. The final RPTEC Tox media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Nota de preparo

Prepare Tox Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-glutamine (G7513), 6 mL RPTEC Tox Supplement (MTOXRTSUP) and 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Tox Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

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