MAK161

Multidrug Resistance Assay Kit

(Fluorometric MDR Assay)

• Sinônimo(s): MDR Assay
• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• uso: (Fluorometric MDR Assay)
• aplicação(ões): pharmaceutical
• método de detecção: fluorometric
• doença(s) relevante(s): cancer
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... ABCB1(5243), ABCC2(1244) ; mouse ... ABCC2(12780) ; rat ... abcc2(25303)

Descrição

Descrição geral

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex = 490/λ_em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Princípio

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex = 490/λ_Em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids