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MAK091

Sigma-Aldrich

Hexokinase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Hexokinase Detection Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detecção

colorimetric

doença(s) relevante(s)

cancer; neurological disorders; hematological disorder

temperatura de armazenamento

−20°C

Informações sobre genes

human ... GCK(2645) , HK1(3098) , HK2(3099) , HK3(3101)
mouse ... GCK(103988) , HK1(15275) , HK2(15277) , HK3(212032)
rat ... GCK(24385) , HK1(25058) , HK2(25059) , HK3(25060)

Descrição geral

Hexokinases, which catalyze the ATP-dependent phosphorylation of aldo- and keto-hexoses to hexose-6-phosphate, catalyze the first step in a number of important biochemical pathways. In mammalian tissues, glucose is the predominant substrate for the hexokinases. There are four isozymes of hexokinase in mammalian tissue, (HK-I, -II, -III, and -IV), which differ in their kinetic and regulatory properties as well as tissue distribution and cofactor use. The hexokinases catalyze the first step in most of the relevant glucose metabolism pathways. Alterations in hexokinase activity are associated with multiple disorders such as X-linked muscular dystrophy, hemolytic anermias, and cancer.

Aplicação

Hexokinase Colorimetric Assay Kit has been used to measure the activity of hexokinase.

Adequação

Suitable for the measurement of Hexokinase activity, analysis of glucose metabolism and cell signaling, and screening anti-diabetic drugs in serum, animal tissues (liver, heart, kidney etc.), and cell culture (adherent or suspension cell).

Princípio

The Hexokinase Colorimetric Assay kit provides a simple and direct procedure for measuring hexokinase activity in a variety of samples. Hexokinase activity is determined by a coupled enzyme assay, in which glucose is converted to glucose-6-phosphate by hexokinase, which is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the hexokinase activity present. One unit of HK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

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Certificados de análise (COA)

Lot/Batch Number
7E21K07890
6L19K07890
6B05K07890
5M12K07890
5B26K07890

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Crystal structures of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
Nishimasu H, et al.
The Journal of Biological Chemistry, 282(13), 9923-9931 (2007)
Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition.
Vivek P, et al.
Indian J. Agri. Sci., 88(2), 222-228 (2018)
Hexokinase II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy.
Roberts D J and Miyamoto S
Cell Death and Differentiation, 22'(2), 248-248 (2015)
Samuel E Schriner et al.
Journal of medicinal food, 19(3), 318-323 (2016-03-18)
The root and rhizome extract of Rhodiola rosea has been extensively used in traditional medicine to improve physical and mental performance and to protect against stress. We, and others, have reported that R. rosea can extend lifespan in flies, worms
Md Wasim Khan et al.
Biochimica et biophysica acta. Molecular basis of disease, 1865(3), 678-687 (2018-12-14)
Hexokinase domain containing 1, a recently discovered putative fifth hexokinase, is hypothesized to play key roles in glucose metabolism. Specifically, during pregnancy in a recent genome wide association study (GWAS), a strong correlation between HKDC1 and 2-h plasma glucose in
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