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Key Documents

MAK051

Sigma-Aldrich

Aconitase Activity Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Aconitase Enzyme Activity Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detecção

colorimetric

doença(s) relevante(s)

neurological disorders; cardiovascular diseases; cancer

temperatura de armazenamento

2-8°C

Informações sobre genes

Descrição geral

The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.

Aplicação

Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the measurement of aconitase activity in biological samples including tissue and cells

Princípio

The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.

substituído por

Nº do produto
Descrição
Preços

Pictogramas

Health hazardCorrosion

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


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