MAK040
Adipogenesis Kit
sufficient for 100 colorimetric or fluorometric tests
Sinônimo(s):
Triglyceride Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.25
Produtos recomendados
uso
sufficient for 100 colorimetric or fluorometric tests
método de detecção
colorimetric
fluorometric
doença(s) relevante(s)
endocrinological disorders, diabetes; cardiovascular diseases; cancer
temperatura de armazenamento
−20°C
Descrição geral
Adipogenesis is the differentiation of pre-adipocytes derived from pluripotent mesenchymal cells into mature adipocytes. Adipocytes, which are capable of storing large quantities of lipids as triglycerides, are the main cells for lipid storage in animals. Adipose tissue plays a critical role in the regulation of whole-body energy homeostasis and adipocyte dysfunction has been implicated in pathological states such as cancer, type II diabetes, cardiovascular disease, and atherosclerosis.
Omental adipocytes are known to promote the invasion and progression of cancer cells especially, ovarian cancer. Close proximity of tumor cells and adipocytes, causes a phenotypic change resulting in the formation of adipocyte-derived fibroblasts, which directly promotes tumor progression. Elevated level of adiponectin produced by adipocytes is observed in heart failure patients, cardiovascular disease, and atherosclerosis. Excess cytokines such as TNFα (tumor necrosis factor -α) in adipocytes results in chronic inflammatory state and inhibits the process of adipogenesis. Adipocytes are known to maintain glucose homeostasis. Adipocytes regulates the entry of fatty acids into circulation and skeletal muscle. This action of adipocytes is associated with the development of type 2 diabetes, which indicates insulin resistance as a result of high levels of fatty acids in the blood.
Omental adipocytes are known to promote the invasion and progression of cancer cells especially, ovarian cancer. Close proximity of tumor cells and adipocytes, causes a phenotypic change resulting in the formation of adipocyte-derived fibroblasts, which directly promotes tumor progression. Elevated level of adiponectin produced by adipocytes is observed in heart failure patients, cardiovascular disease, and atherosclerosis. Excess cytokines such as TNFα (tumor necrosis factor -α) in adipocytes results in chronic inflammatory state and inhibits the process of adipogenesis. Adipocytes are known to maintain glucose homeostasis. Adipocytes regulates the entry of fatty acids into circulation and skeletal muscle. This action of adipocytes is associated with the development of type 2 diabetes, which indicates insulin resistance as a result of high levels of fatty acids in the blood.
Aplicação
Adipogenesis Kit has been used to study the intramyocellular triglyceride accumulation in myotubes. It has been used to study the effect of NS5ABP37 on hepatocellular carcinoma by determining the total intracellular triglyceride.
Adequação
Suitable for the quantification of triglyceride accumulation in cells and tissue
Princípio
In this kit, total cellular and tissue concentrations of triglycerides are determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the triglycerides present. This kit is able to detect triglycerides in as few as 1 × 104 differentiated 3T3 L1 cells.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
188.6 °F - closed cup
Ponto de fulgor (°C)
87 °C - closed cup
Certificados de análise (COA)
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Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Shenghu Feng et al.
Cancer science, 108(1), 12-22 (2016-11-20)
The molecular mechanism underlying non-alcoholic fatty liver disease progression to hepatocellular carcinoma (HCC) remains unknown. In this study, immunohistochemistry staining results showed that NS5ABP37 protein, which is in a state of lower expression in tumor tissues, decreased with increasing degree
Adipocyte-Derived Fibroblasts promote tumor progression and contribute to desmoplastic reaction in breast cancer.
Bochet L, et al.
Cancer Research, 530-530 (2013)
Defining the role of DAG, mitochondrial function and lipid deposition in palmitate-induced proinflammatory signalling and its counter-modulation by palmitoleate.
Macrae K, et al.
Journal of Lipid Research, M036996-M036996 (2013)
A new role for the natriuretic peptides: metabolic regulators of the adipocyte.
Costello-Boerrigter L C and Burnett J C
Journal of the American College of Cardiology (2009)
Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Nieman K M, et al.
Nature Medicine, 17(11), 1498-1498 (2011)
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