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MAK013

Sigma-Aldrich

Glucose and Sucrose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinônimo(s):

Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

aplicação(ões)

cosmetics
food and beverages

método de detecção

colorimetric
fluorometric

doença(s) relevante(s)

endocrinological disorders, diabetes

temperatura de armazenamento

−20°C

Descrição geral

Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.

Aplicação

Glucose and sucrose assay kit has been used to:
  • determine sucrose, aerobic glycolysis and pyruvate kinase activity
  • determine protein and sugar in strawberries
  • determine plasma insulin and glucose levels in mice
  • analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.

Adequação

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Princípio

In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334,H412

Declarações de precaução

P261 - P273 - P284 - P501

Classificações de perigo

Aquatic Chronic 3 - Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup

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Certificados de análise (COA)

Lot/Batch Number
8A20K06160
7E12K06160
6K06K06160
6D24K06160
5C12K06160

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Protocolos

Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)

Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.

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