M6697
Anti-MLKL (58-70) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinônimo(s):
Anti-mixed lineage kinase domain-like (Homo sapiens)
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
IgG fraction of antiserum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
forma
buffered aqueous solution
peso molecular
antigen ~54 kDa
reatividade de espécies
human
técnica(s)
western blot: 1:250-1:500
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... MLKL(197259)
Descrição geral
MLKL (mixed lineage kinase domain-like) is a protein that belongs to the protein kinase superfamily. It facilitates necrosis signaling downstream of the kinase RIP3 (receptor-interacting serine-threonine kinase 3) . Anti-MLKL (58-70) antibody can be used in western blotting. Rabbit anti- MLKL (58-70) antibody reacts specifically with MLKL protein.
MLKL is encoded by the gene mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane.
Imunogênio
synthetic peptide corresponding to amino acids 58-70 of human MLKL.
Aplicação
Anti-MLKL (58-70) antibody produced in rabbit has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunocytochemistry (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
western blot: 1:250-1:500
Ações bioquímicas/fisiológicas
Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase-dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis, which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Sara R Oliveira et al.
Cell death discovery, 4, 10-10 (2018-08-01)
Necroptosis is a regulated form of necrosis, which may be critical in the pathogenesis of neurodegenerative diseases. Neuroinflammation, characterized by the activation of glial cells such as microglia, is closely linked with neurodegenerative pathways and constitutes a major mechanism of
Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis.
Rodriguez DA, et al.
Cell Death and Differentiation, 23(1), 76-76 (2016)
Tommaso Selmi et al.
BMC cancer, 15, 357-357 (2015-05-06)
ZFP36 is an mRNA binding protein that exerts anti-tumor activity in glioblastoma by triggering cell death, associated to an increase in the stability of the kinase RIP1. We used cell death assays, size exclusion chromatography, Co-Immunoprecipitation, shRNA lentivectors and glioma
ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly.
Selmi T, et al.
BMC Cancer, 15(1), 357-357 (2015)
MLKL activation triggers NLRP3-mediated processing and release of IL-β independently of gasdermin-D
Gutierrez KD, et al.
Journal of Immunology, 47(1), 1601757-1601757 (2017)
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