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Documentos Principais

L5158

Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Sinônimo(s):

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

Nome do produto

Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

recombinante

expressed in E. coli

Nível de qualidade

Ensaio

≥95% (SDS-PAGE)

Formulário

buffered aqueous solution

qualidade

endotoxin tested

técnica(s)

cell culture | mammalian: suitable

temperatura de armazenamento

2-8°C

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Aplicação

Leukemia Inhibitory Factor from mouse has been used:
  • as a component of enriched co-culture media
  • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
  • as a component in maintenance medium for maintaining embryonic stem cells
  • to treat ovaries in ovarian culture

Ações bioquímicas/fisiológicas

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

forma física

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Nota de análise

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Informações legais

TWEEN is a registered trademark of Croda International PLC

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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D P Gearing et al.
The EMBO journal, 6(13), 3995-4002 (1987-12-20)
Leukaemia inhibitory factor (LIF) can induce macrophage differentiation in M1 murine myeloid leukaemic cells and suppress their proliferation in vitro. It does not stimulate the proliferation of normal progenitor cells and is apparently distinct from known colony-stimulating factors. We have

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