HPA021578
Anti-TRIM65 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Sinônimo(s):
TRIM65 Antibody - Anti-TRIM65 antibody produced in rabbit, Trim65 Antibody, Anti-Tripartite motif-containing protein 65
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About This Item
Produtos recomendados
fonte biológica
rabbit
conjugado
unconjugated
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
linha de produto
Prestige Antibodies® Powered by Atlas Antibodies
forma
buffered aqueous glycerol solution
reatividade de espécies
human
técnica(s)
immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
sequência de imunogênio
LELFCRTEGRCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAEGQLLELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRS
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... TRIM65(201292)
Descrição geral
Tripartite motif 65 (TRIM65) belongs to TRIM family proteins. TRIM family contains B-box 1 and a B-box 2 type domain. The coiled domain possesses hyper-helical regions. TRIM65 exhibits a SPRY (spla and ryanodine) domain at its C-terminus. Two anti-parallel β-sheets in the SPRY domain form a curved β-sandwich. Trim65 homologous genes have been found in mammals.
Imunogênio
Tripartite motif-containing protein 65 recombinant protein epitope signature tag (PrEST)
Aplicação
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Ações bioquímicas/fisiológicas
Ubiquitination of TNRC6 proteins is through E3 ligase activity of TRIM65. The coiled coil domain plays a role in protein-protein interactions and oligomerization. It is associated with repression of miRNA activity. TRIM65 colludes and adjoins with trinucleotide repeat TNRC6 (trinucleotide repeat-containing gene 6) proteins. Genomic studies on cerebral white matter lesions have found a new locus on chromosome 17q25 accommodating TRIM65. The studies also determine a probable mechanism leading to these lesions. Uncontrolled TRIM65 activities extirpate TNRC6 stability and alter RISC (RNA-induced silencing complex) stability, miRNA activity and cellular homeostasis. Gene silencing of TRIM65 with that of siRNA leads to attenuation of let-7a reporter activity. An abnormal expression of TRIM65 has a similar effect.
Características e benefícios
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Ligação
Corresponding Antigen APREST75679
forma física
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Informações legais
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity.
Rajsbaum R
Journal of Molecular Biology, 426(6), 1265-1284 (2014)
TRIM family proteins and their emerging roles in innate immunity.
Ozato K
Nature Reviews: Immunology, 8(11), 849-860 (2008)
TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
Li S
Proceedings of the National Academy of Sciences of the USA, 111(19), 6970-6975 (2014)
Emanuela Marchese et al.
International journal of molecular sciences, 23(16) (2022-08-27)
Bardet-Biedl syndrome (BBS) is a rare autosomal recessive ciliopathy resulting in multiple organ dysfunctions, including chronic kidney disease (CKD). Despite the recent progress in the 'ciliopathy' field, there is still little information on the mechanisms underlying renal disease. To elucidate
Trim65: a cofactor for regulation of the microRNA pathway.Li
Li s
RNA Biology, 11(9), 1113-1121 (2014)
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