HPA018481

Anti-SEPT2 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Sinônimo(s): Anti-Protein NEDD5, Anti-Septin-2
• Código UNSPSC: 12352203
• Número do Atlas de Proteínas Humanas: HPA018481
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• linha de produto: Prestige Antibodies^® Powered by Atlas Antibodies
• Formulário: buffered aqueous glycerol solution
• reatividade de espécies: human, mouse, rat; mouse, human, rat
• validação aprimorada: RNAi knockdown; Learn more about Antibody Enhanced Validation
• técnica(s): immunoblotting: 0.04-0.4 μg/mL; immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:200-1:500
• sequência de imunogênio: WGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGH
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SEPT2(4735)

Descrição

Descrição geral

The gene septin-2 (SEPT2) is mapped to human chromosome 2q37. It belongs to a conserved family of guanosine triphosphate binding proteins required for cell division. SEPT2 is present at the mid-body, the cleavage furrow and the central spindle of cells undergoing cytokinesis. In metaphase cells, SEPT2 is present within the microtubule spindle. SEPT2 is widely expressed with highest levels in brain tissues.

Imunogênio

Septin-2 recombinant protein epitope signature tag (PrEST)

Aplicação

Anti-SEPT2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Ações bioquímicas/fisiológicas

At metaphase septin-2 (SEPT2) is crucial for maintaining CENP-E (Centromere-associated protein E) at kinetochores, required for chromosome congression. In anaphase, SEPT2 is needed for chromosome segregation and spindle elongation. SEPT2 forms a complex with SEPT7 and SEPT9. All three together are present along the length of the axoneme in the primary cilium of retinal pigmented epithelial cells. The complex is crucial for ciliogenesis. SEPT2 is recruited to the site of bacterial entry next to actin and is involved in bacterial invasion of Listeria and Shigella. SEPT2 is up-regulated in human hepatoma carcinoma cells (HCC). Casein kinase-2 mediated phosphorylation of SEPT2 is important for proliferation of HCC.

Características e benefícios

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Ligação

Corresponding Antigen APREST72699

forma física

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informações legais

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)